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hsv1 icp4  (Abcam)


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    Structured Review

    Abcam hsv1 icp4
    HSV-P10 construction and characterization. a Graphical representation of PTEN and PTENα coding sequences showing the mutations engineered in PTENα start codons to enhance translation of full-length PTENα. b – c Graphical representation of the DNA structure of wild-type F-strain <t>HSV1</t> backbone showing doubly deleted γ34.5 genes, and gene-disrupting insertional ICP6 locus containing an enhanced green fluorescent protein (eGFP) gene with PTENα insertion (HSV-P10) or without PTENα (HSVQ). d PTENα production in infected tumor cell lysates over time. The indicated tumor cells were infected with HSV-P10 at MOI = 0.5 and harvested 0–12 hpi and probed for PTEN by western blot
    Hsv1 Icp4, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 874 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hsv1+icp4/Anti-GAPDH+antibody+%5BmAbcam+9484%5D+-+Loading+Control/pmc06258708-292-11-10
    Average 99 stars, based on 874 article reviews
    hsv1 icp4 - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "PTEN expression by an oncolytic herpesvirus directs T-cell mediated tumor clearance"

    Article Title: PTEN expression by an oncolytic herpesvirus directs T-cell mediated tumor clearance

    Journal: Nature Communications

    doi: 10.1038/s41467-018-07344-1

    HSV-P10 construction and characterization. a Graphical representation of PTEN and PTENα coding sequences showing the mutations engineered in PTENα start codons to enhance translation of full-length PTENα. b – c Graphical representation of the DNA structure of wild-type F-strain HSV1 backbone showing doubly deleted γ34.5 genes, and gene-disrupting insertional ICP6 locus containing an enhanced green fluorescent protein (eGFP) gene with PTENα insertion (HSV-P10) or without PTENα (HSVQ). d PTENα production in infected tumor cell lysates over time. The indicated tumor cells were infected with HSV-P10 at MOI = 0.5 and harvested 0–12 hpi and probed for PTEN by western blot
    Figure Legend Snippet: HSV-P10 construction and characterization. a Graphical representation of PTEN and PTENα coding sequences showing the mutations engineered in PTENα start codons to enhance translation of full-length PTENα. b – c Graphical representation of the DNA structure of wild-type F-strain HSV1 backbone showing doubly deleted γ34.5 genes, and gene-disrupting insertional ICP6 locus containing an enhanced green fluorescent protein (eGFP) gene with PTENα insertion (HSV-P10) or without PTENα (HSVQ). d PTENα production in infected tumor cell lysates over time. The indicated tumor cells were infected with HSV-P10 at MOI = 0.5 and harvested 0–12 hpi and probed for PTEN by western blot

    Techniques Used: Infection, Western Blot

    HSV-P10 induces enhanced immune cell influx towards infected tumors. Tumor-bearing FVB/N mice were treated with 1e5 pfu of HSVQ, HSV-P10, or saline control 8 days post tumor implantation. a Stitched 4X bright field images of H&E stained sagittal sections of mouse brains 3 days post virus injection. b Representative 20X bright field images of H&E, Keratin 8, F4/80, NKp46, CD3, CD8α, PD-L1, and HSV1 (GFP) from sagittal sections of mouse brains 3 days post virus injection. Scale = 0.1 mm. c Ratio of macrophages (CD11b + F4/80 + CD45 bright ) to microglia (CD11b + F4/80 + CD45 dim ). d Percentage of cells in c positive for MHC-II. e Dendritic cell (CD11c + CD80 + MHC-II + ) infiltration. f NK cell (CD49b + NKp46 + ) infiltration. g CD8 + T-cell (CD3 + CD4 + ) infiltration. h CD4 + T-cell (CD3 + CD8 + ) infiltration. Data shown are averages ± s.d ( n = 3/group) Statistical significance was assessed by one-way ANAOVA ( n = 3, * p < 0.05, *** p < 0.001, **** p < 0.0001). Gating strategies for ( c – h ) are decribed in supplementary figure
    Figure Legend Snippet: HSV-P10 induces enhanced immune cell influx towards infected tumors. Tumor-bearing FVB/N mice were treated with 1e5 pfu of HSVQ, HSV-P10, or saline control 8 days post tumor implantation. a Stitched 4X bright field images of H&E stained sagittal sections of mouse brains 3 days post virus injection. b Representative 20X bright field images of H&E, Keratin 8, F4/80, NKp46, CD3, CD8α, PD-L1, and HSV1 (GFP) from sagittal sections of mouse brains 3 days post virus injection. Scale = 0.1 mm. c Ratio of macrophages (CD11b + F4/80 + CD45 bright ) to microglia (CD11b + F4/80 + CD45 dim ). d Percentage of cells in c positive for MHC-II. e Dendritic cell (CD11c + CD80 + MHC-II + ) infiltration. f NK cell (CD49b + NKp46 + ) infiltration. g CD8 + T-cell (CD3 + CD4 + ) infiltration. h CD4 + T-cell (CD3 + CD8 + ) infiltration. Data shown are averages ± s.d ( n = 3/group) Statistical significance was assessed by one-way ANAOVA ( n = 3, * p < 0.05, *** p < 0.001, **** p < 0.0001). Gating strategies for ( c – h ) are decribed in supplementary figure

    Techniques Used: Infection, Tumor Implantation, Staining, Injection

    Related Articles

    Infection:

    Article Title: PTEN expression by an oncolytic herpesvirus directs T-cell mediated tumor clearance
    Article Snippet: PVDF membranes were exposed to antibodies following manufacturer’s protocol.PVDF membranes were exposed to antibodies following manufacturer’s protocol.. Primary (from CST: PTEN (9559), Akt (4691), pAkt-S473 (9271), from abcam: HSV1 ICP4 (ab6514), GAPDH (ab9484)) and secondary antibodies (from abcam: anti-mouse HRP (ab6789), anti-rabbit HRP (ab6721) can be found in Supplementary Data .. All phospho-antibody incubations were performed overnight at 4 °C, and all non-phospho-antibody incubations were performed at RT for 1–2 h. Uncropped blots are depicted in Supplementary Figure .All phospho-antibody incubations were performed overnight at 4 °C, and all non-phospho-antibody incubations were performed at RT for 1–2 h. Uncropped blots are depicted in Supplementary Figure .

    Western Blot:

    Article Title: PTEN expression by an oncolytic herpesvirus directs T-cell mediated tumor clearance
    Article Snippet: PVDF membranes were exposed to antibodies following manufacturer’s protocol.PVDF membranes were exposed to antibodies following manufacturer’s protocol.. Primary (from CST: PTEN (9559), Akt (4691), pAkt-S473 (9271), from abcam: HSV1 ICP4 (ab6514), GAPDH (ab9484)) and secondary antibodies (from abcam: anti-mouse HRP (ab6789), anti-rabbit HRP (ab6721) can be found in Supplementary Data .. All phospho-antibody incubations were performed overnight at 4 °C, and all non-phospho-antibody incubations were performed at RT for 1–2 h. Uncropped blots are depicted in Supplementary Figure .All phospho-antibody incubations were performed overnight at 4 °C, and all non-phospho-antibody incubations were performed at RT for 1–2 h. Uncropped blots are depicted in Supplementary Figure .

    Tumor Implantation:

    Article Title: PTEN expression by an oncolytic herpesvirus directs T-cell mediated tumor clearance
    Article Snippet: PVDF membranes were exposed to antibodies following manufacturer’s protocol.PVDF membranes were exposed to antibodies following manufacturer’s protocol.. Primary (from CST: PTEN (9559), Akt (4691), pAkt-S473 (9271), from abcam: HSV1 ICP4 (ab6514), GAPDH (ab9484)) and secondary antibodies (from abcam: anti-mouse HRP (ab6789), anti-rabbit HRP (ab6721) can be found in Supplementary Data .. All phospho-antibody incubations were performed overnight at 4 °C, and all non-phospho-antibody incubations were performed at RT for 1–2 h. Uncropped blots are depicted in Supplementary Figure .All phospho-antibody incubations were performed overnight at 4 °C, and all non-phospho-antibody incubations were performed at RT for 1–2 h. Uncropped blots are depicted in Supplementary Figure .

    Staining:

    Article Title: PTEN expression by an oncolytic herpesvirus directs T-cell mediated tumor clearance
    Article Snippet: PVDF membranes were exposed to antibodies following manufacturer’s protocol.PVDF membranes were exposed to antibodies following manufacturer’s protocol.. Primary (from CST: PTEN (9559), Akt (4691), pAkt-S473 (9271), from abcam: HSV1 ICP4 (ab6514), GAPDH (ab9484)) and secondary antibodies (from abcam: anti-mouse HRP (ab6789), anti-rabbit HRP (ab6721) can be found in Supplementary Data .. All phospho-antibody incubations were performed overnight at 4 °C, and all non-phospho-antibody incubations were performed at RT for 1–2 h. Uncropped blots are depicted in Supplementary Figure .All phospho-antibody incubations were performed overnight at 4 °C, and all non-phospho-antibody incubations were performed at RT for 1–2 h. Uncropped blots are depicted in Supplementary Figure .

    Injection:

    Article Title: PTEN expression by an oncolytic herpesvirus directs T-cell mediated tumor clearance
    Article Snippet: PVDF membranes were exposed to antibodies following manufacturer’s protocol.PVDF membranes were exposed to antibodies following manufacturer’s protocol.. Primary (from CST: PTEN (9559), Akt (4691), pAkt-S473 (9271), from abcam: HSV1 ICP4 (ab6514), GAPDH (ab9484)) and secondary antibodies (from abcam: anti-mouse HRP (ab6789), anti-rabbit HRP (ab6721) can be found in Supplementary Data .. All phospho-antibody incubations were performed overnight at 4 °C, and all non-phospho-antibody incubations were performed at RT for 1–2 h. Uncropped blots are depicted in Supplementary Figure .All phospho-antibody incubations were performed overnight at 4 °C, and all non-phospho-antibody incubations were performed at RT for 1–2 h. Uncropped blots are depicted in Supplementary Figure .



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    HSV-P10 construction and characterization. a Graphical representation of PTEN and PTENα coding sequences showing the mutations engineered in PTENα start codons to enhance translation of full-length PTENα. b – c Graphical representation of the DNA structure of wild-type F-strain <t>HSV1</t> backbone showing doubly deleted γ34.5 genes, and gene-disrupting insertional ICP6 locus containing an enhanced green fluorescent protein (eGFP) gene with PTENα insertion (HSV-P10) or without PTENα (HSVQ). d PTENα production in infected tumor cell lysates over time. The indicated tumor cells were infected with HSV-P10 at MOI = 0.5 and harvested 0–12 hpi and probed for PTEN by western blot
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    HSV-P10 construction and characterization. a Graphical representation of PTEN and PTENα coding sequences showing the mutations engineered in PTENα start codons to enhance translation of full-length PTENα. b – c Graphical representation of the DNA structure of wild-type F-strain <t>HSV1</t> backbone showing doubly deleted γ34.5 genes, and gene-disrupting insertional ICP6 locus containing an enhanced green fluorescent protein (eGFP) gene with PTENα insertion (HSV-P10) or without PTENα (HSVQ). d PTENα production in infected tumor cell lysates over time. The indicated tumor cells were infected with HSV-P10 at MOI = 0.5 and harvested 0–12 hpi and probed for PTEN by western blot
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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cell

    Article Title: Phosphoinositide Interactions Position cGAS at the Plasma Membrane to Ensure Efficient Distinction between Self- and Viral DNA

    doi: 10.1016/j.cell.2019.01.049

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Mouse monoclonal anti-HSV1 ICP4 antibody , Virusys , Cat# P1101.

    Techniques: Microscopy, Western Blot, Virus, Modification, Recombinant, Protease Inhibitor, Electron Microscopy, Staining, Cell Viability Assay, Luciferase, Expressing, Software, Size-exclusion Chromatography

    HSV-P10 construction and characterization. a Graphical representation of PTEN and PTENα coding sequences showing the mutations engineered in PTENα start codons to enhance translation of full-length PTENα. b – c Graphical representation of the DNA structure of wild-type F-strain HSV1 backbone showing doubly deleted γ34.5 genes, and gene-disrupting insertional ICP6 locus containing an enhanced green fluorescent protein (eGFP) gene with PTENα insertion (HSV-P10) or without PTENα (HSVQ). d PTENα production in infected tumor cell lysates over time. The indicated tumor cells were infected with HSV-P10 at MOI = 0.5 and harvested 0–12 hpi and probed for PTEN by western blot

    Journal: Nature Communications

    Article Title: PTEN expression by an oncolytic herpesvirus directs T-cell mediated tumor clearance

    doi: 10.1038/s41467-018-07344-1

    Figure Lengend Snippet: HSV-P10 construction and characterization. a Graphical representation of PTEN and PTENα coding sequences showing the mutations engineered in PTENα start codons to enhance translation of full-length PTENα. b – c Graphical representation of the DNA structure of wild-type F-strain HSV1 backbone showing doubly deleted γ34.5 genes, and gene-disrupting insertional ICP6 locus containing an enhanced green fluorescent protein (eGFP) gene with PTENα insertion (HSV-P10) or without PTENα (HSVQ). d PTENα production in infected tumor cell lysates over time. The indicated tumor cells were infected with HSV-P10 at MOI = 0.5 and harvested 0–12 hpi and probed for PTEN by western blot

    Article Snippet: Primary (from CST: PTEN (9559), Akt (4691), pAkt-S473 (9271), from abcam: HSV1 ICP4 (ab6514), GAPDH (ab9484)) and secondary antibodies (from abcam: anti-mouse HRP (ab6789), anti-rabbit HRP (ab6721) can be found in Supplementary Data .

    Techniques: Infection, Western Blot

    HSV-P10 induces enhanced immune cell influx towards infected tumors. Tumor-bearing FVB/N mice were treated with 1e5 pfu of HSVQ, HSV-P10, or saline control 8 days post tumor implantation. a Stitched 4X bright field images of H&E stained sagittal sections of mouse brains 3 days post virus injection. b Representative 20X bright field images of H&E, Keratin 8, F4/80, NKp46, CD3, CD8α, PD-L1, and HSV1 (GFP) from sagittal sections of mouse brains 3 days post virus injection. Scale = 0.1 mm. c Ratio of macrophages (CD11b + F4/80 + CD45 bright ) to microglia (CD11b + F4/80 + CD45 dim ). d Percentage of cells in c positive for MHC-II. e Dendritic cell (CD11c + CD80 + MHC-II + ) infiltration. f NK cell (CD49b + NKp46 + ) infiltration. g CD8 + T-cell (CD3 + CD4 + ) infiltration. h CD4 + T-cell (CD3 + CD8 + ) infiltration. Data shown are averages ± s.d ( n = 3/group) Statistical significance was assessed by one-way ANAOVA ( n = 3, * p < 0.05, *** p < 0.001, **** p < 0.0001). Gating strategies for ( c – h ) are decribed in supplementary figure

    Journal: Nature Communications

    Article Title: PTEN expression by an oncolytic herpesvirus directs T-cell mediated tumor clearance

    doi: 10.1038/s41467-018-07344-1

    Figure Lengend Snippet: HSV-P10 induces enhanced immune cell influx towards infected tumors. Tumor-bearing FVB/N mice were treated with 1e5 pfu of HSVQ, HSV-P10, or saline control 8 days post tumor implantation. a Stitched 4X bright field images of H&E stained sagittal sections of mouse brains 3 days post virus injection. b Representative 20X bright field images of H&E, Keratin 8, F4/80, NKp46, CD3, CD8α, PD-L1, and HSV1 (GFP) from sagittal sections of mouse brains 3 days post virus injection. Scale = 0.1 mm. c Ratio of macrophages (CD11b + F4/80 + CD45 bright ) to microglia (CD11b + F4/80 + CD45 dim ). d Percentage of cells in c positive for MHC-II. e Dendritic cell (CD11c + CD80 + MHC-II + ) infiltration. f NK cell (CD49b + NKp46 + ) infiltration. g CD8 + T-cell (CD3 + CD4 + ) infiltration. h CD4 + T-cell (CD3 + CD8 + ) infiltration. Data shown are averages ± s.d ( n = 3/group) Statistical significance was assessed by one-way ANAOVA ( n = 3, * p < 0.05, *** p < 0.001, **** p < 0.0001). Gating strategies for ( c – h ) are decribed in supplementary figure

    Article Snippet: Primary (from CST: PTEN (9559), Akt (4691), pAkt-S473 (9271), from abcam: HSV1 ICP4 (ab6514), GAPDH (ab9484)) and secondary antibodies (from abcam: anti-mouse HRP (ab6789), anti-rabbit HRP (ab6721) can be found in Supplementary Data .

    Techniques: Infection, Tumor Implantation, Staining, Injection